Newsportal - Ruhr-Universität Bochum
Structure and function of photosynthesis protein explained in detail
An international team of researchers has solved the structure and elucidated the function of photosynthetic complex I. This membrane protein complex plays a major role in dynamically rewiring photosynthesis. The team from the Max Planck Institute for Biochemistry, Osaka University and Ruhr-Universität Bochum together with their collaboration partners report the work in the journal “Science”, published online on 20 December 2018. “The results close one of the last major gaps in our understanding of photosynthetic electron transport pathways,” says Associate Professor Dr. Marc Nowaczyk, who heads the Bochum project group “Cyanobacterial Membrane Protein Complexes”.
Biology’s electrical circuits
Complex I is found in most living organisms. In plant cells it is used in two places: one is in mitochondria, the cell’s power plants, the other is in chloroplasts, where photosynthesis occurs. In both instances, it forms part of an electron transport chain, which can be thought of as biology’s electrical circuit. These are used to drive the cells molecular machines responsible for energy production and storage. The structure and function of mitochondrial complex I as part of cellular respiration has been well investigated, whereas photosynthetic complex I has been little studied so far.
Using cryoelectron microscopy, the researchers were able to solve for the first time the molecular structure of photosynthetic complex I. They showed that it differs considerably from its respiratory relative. In particular, the part responsible for electron transport has a different structure, since it is optimised for cyclic electron transport in photosynthesis.
Cyclic electron transport represents a molecular short circuit in which electrons are reinjected into the photosynthetic electron transport chain instead of being stored. Marc Nowaczyk explains: “The molecular details of this process have been unknown and additional factors have not yet been unequivocally identified.” The research team simulated the process in a test tube and showed that the protein ferredoxin plays a major role. Using spectroscopic methods, the scientists also demonstrated that the electron transport between ferredoxin and complex I is highly efficient.
Molecular fishing rod
In the next step, the group analysed at the molecular level which structural elements are responsible for the efficient interaction of complex I and ferredoxin. Further spectroscopic measurements showed that complex I has a particularly flexible part in its structure, which captures the protein ferredoxin like a fishing rod. This allows ferredoxin to reach the optimal binding position for electron transfer.
“This enabled us to bring the structure together with the function of the photosynthetic complex I and gain a detailed insight into the molecular basis of electron transport processes,” summarises Marc Nowaczyk. “In the future, we plan to use this knowledge to create artificial electron transport chains that will enable new applications in the field of synthetic biology.”
- Max Planck Institute for Biochemistry
- Max Planck Institute for Chemical Energy Conversion
- Yokohama City University
- Australian National University
- Ludwig-Maximilians-Universität München
- Université Paris-Saclay
- Osaka University
- Ruhr-Universität Bochum
The German Research Foundation supported the study within the framework of the Cluster of Excellence Resolv (EXC 1069), the research group FOR2092 (EN 1194/1-1 and NO 836/3-2), the priority programme 2002 (NO 836/4-1) and the project NO 836/1-1. Further funding came from the Max Planck Society, the Australian Research Council (FT140100834, N.C.), JST-CREST (JPMJCR13M4, G.K.), MEXT-KAKENHI (16H06560, G.K.), the French Infrastructure for Integrated Structural Biology (FRISBI ANR-10-INSB-05) and the International Joint Research Promotion Program of Osaka University.
Jan. M. Schuller et al.: Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer, in: Science, 2018, DOI: 10.1126/science.aau3613
PD Dr. Marc Nowaczyk
Department of Plant Biochemistry
Faculty of Biology and Biotechnology
Phone: +49 234 32 23657
21 December 2018