For the first time Mehdi Behroozi, Felix Ströckens and Xavier Helluy (from the left) examined a cold-blooded reptile using functional MRI. © RUB, Marquard

Biopsychology Crocodiles Listen to Classical Music in MRI Scanner

Imaging technology has been successfully deployed to record activities of a reptilian brain for the first time.

What happens in a crocodile’s brain when it hears complex sounds? An international research team headed by Dr Felix Ströckens from the Department of Biopsychology at Ruhr-Universität Bochum (RUB) has provided the answer to this question. In a first, the researchers examined a cold-blooded reptile using functional MRI. They were thus able to determine that complex stimuli triggered activation patterns in the crocodile’s brain that are similar to those in birds and mammals – a deep insight into evolution. The results were published in the journal “Proceedings of the Royal Society B: Biological Sciences” on April 25, 2018.

Link to dinosaurs

Crocodiles count among the most ancient species of vertebrates and have barely changed over the space of more than 200 million years. Accordingly, they constitute a link between dinosaurs and bird species today. “Analyses of crocodile brains thus provide deep insights into the evolution of the nervous system in mammals and may help us understand at which point certain brain structures and behaviours associated therewith were formed,” explains Felix Ströckens.

Overcoming technical obstacles

The objective pursued by the team of researchers from the Iran, South Africa, France, and Germany was to study Nile crocodiles and to ascertain the way sensory information is processed in their brain. To this end, they deployed functional MRI (fMRI) – a method that is routinely used in clinical diagnostics and research, but which has never yet been utilised to study a cold-blooded reptile. “In the first step, we had to overcome a number of technical obstacles,” says research team member Mehdi Behroozi. “For example, we had to adjust the scanner to the crocodile’s physiology, which differs massively from that of mammals in several aspects.”

Astonishing similarity of patterns

Subsequently, the researchers exposed the animals to various visual and auditory stimuli, including classical music by Johann Sebastian Bach. At the same time, they measured the animals’ brain activity. The results have shown that additional brain areas are activated during exposure to complex stimuli such as classical music – as opposed to exposure to simple sounds. The processing patterns strongly resemble the patterns identified in mammals and birds in similar studies.

Processing patterns formed at an early stage

Consequently, the researchers assume that fundamental neuronal processing mechanisms of sensory stimuli formed at an early evolutionary stage and that they can be traced back to the same origins in all vertebrates.

By successfully deploying fMRI for the examination of a reptile for the first time worldwide, the researchers, moreover, demonstrated that the method does work for poikilothermic organisms. This non-invasive technology can thus be used for many other species that have not yet been studied in depth.

Funding

Funded by the National Research Foundation of South Africa, Thuthuka Grant (TTK14051567366), the German Research Foundation (Gu227/16-1) and the Collaborative Research Centre 874.

Original publication

Mehdi Behroozi, Brendon K. Billings, Xavier Helluy, Paul R. Manger, Onur Güntürkün, Felix Ströckens: Functional MRI in the Nile crocodile: a new avenue for evolutionary neurobiology, in: Proceedings of the Royal Society B: Biological Sciences, 2018, DOI: 0.1098/rspb.2018.0178

Press contact

Dr Felix Ströckens
Institute of Cognitive Neuroscience
Department of Biopsychology
Faculty of Psychology
Ruhr-Universität Bochum
Phone: 0234 32 26845
Email: felix.stroeckens@rub.de

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Published

Thursday
03 May 2018
9:14 am

By

Meike Drießen

Translated by

Donata Zuber

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